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KMID : 0391319930030010015
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Korean Journal of Biological Response Modifiers
1993 Volume.3 No. 1 p.15 ~ p.22
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Effect of Various Extrinsic and Intrinsic Factors on the Nitric Oxide Production of Murine Macrophages
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최혜심
전창덕/이복수/이병순/박석돈/오종석/정헌택
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Abstract
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To investigate the capacity of cytokines and biological response modifiers (BRMs) to induce nitricoxide from macrophages, we isolated the resident peritoneal macrophages from Balb/c mouse (7 to 10 week old) and incubated for 24~48h with the
various
cytokines and BRMs. After incubation, the nitrite conteentration in the medium was measured spectrophotometrically by a microplate assay method.
Elevated NO release was observed in resident peritoneal macrophages after a 48h incubation with gamma-interferon (γ-IFN). A23187, and H-7. Incubation of macrophages with γ-IFN for 48h in the p0resence of lipoplolysaccharide (LPS) augmented NO
release
in dose dependent manner.
Although TNF-αdid not induce NO synthesis on its own, the cytokine with γ-IFN increased macrophage NO production up to six fold over that in macrophage treated with γ-IFN alone. A23187 also showed priming effect on the NO release, when
incubated
with
LPS. Whereas, anti-TNF-α antibody or antisense TNF-α oligodeoxynucleotide inhibited the release of NO by macrophage induced by γ-IFN and LPS.
According to the above data, γ-IFN, A231878 or H-7 was shown to be capable of inducing NO release as a sole agnet, and especially A23187 could mimic γ-IFN induced macrophage activation for the induction of L-arginine dependent NO release. Also
either
r-LPS or A23187 and LPS could interact synergistically to induce NO release from macrophages.
Because the anti-tumoral and anti-microbial activities of macrophage are known to be dependent on the production of NO by macrophages, the above data suggest that evaluations of BRM for the induction of NO producition by macrophages are important
for
clinical application.
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KEYWORD
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